This estimator helps with determining the reagents and sequencing runs that are needed to arrive at the desired coverage for your experiment. Perform the following steps to run the estimator:
Click the button for the type of application.
Select the application or product from the dropdown menu.
Enter the input parameters in the open fields. The gray fields are locked, indicating that the value cannot be changed for that application or product.
Select the instruments you want to perform the estimation for.
The estimator writes a table containing the number of lanes or flow cells you need to use for the desired product and parameters. You can also download the results in a comma-separated values file, so you can share data or use the tables in Excel.
Output is an estimation based on recommended cluster density and read length; real output will vary.
The estimator uses an estimate of clusters passing filter commonly found for balanced genomes (such as PhiX or the human genome). If you plan to sequence an unbalanced genome, you may have a lower number of clusters passing filter, and consequently a lower output per lane.