This calculator helps with determining the reagents and sequencing runs that are needed to arrive at the desired coverage for your experiment. Perform the following steps to run the calculator:

  1. Click the button for DNA or RNA applications from.
  2. Select the application or product from the dropdown menu.
  3. Enter the input parameters in the open fields. The gray fields are locked, indicating that the value cannot be changed for that application or product.
  4. Select the instruments you want to perform the calculation for.
  5. Click Submit.

The calculator writes a table containing the number of lanes or flow cells you need to use for the desired product and parameters. You can also download the results in a comma-separated values file, so you can share data or use the tables in Excel.

Additional Considerations